| Page | Description | Tags |
| AatII |
Recognition [GACGTC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X
* Restriction enzyme
* Buffer for restriction enzymes
* REBA… |
Restriction enzyme,
3'sticky |
| AccI |
Recognition [GTMKAC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X
* GTATAC / GTCGAC
* M,K
* Restriction enzyme
* HincII
*… |
Restriction enzyme,
5'sticky |
| AluI |
Recognition [AGCT] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE
… |
Restriction enzyme,
blunt |
| ApaI |
Recognition [GGGCCC] Temp. 25 ºC BufferLowMidHighAcKCl ≥75% O X X O X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE… |
Restriction enzyme,
3'sticky,
Not 37°C reaction |
| AvaI |
Recognition [CYCGRG] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X
* GTCGAG / CCCGGG
* R,Y
* Restriction enzyme
* SmaI
* Bu… |
restriction enzyme,
5'sticky |
| AvaII |
Recognition [GGWCC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X
* GGACC / GGTCC
* W
* Restriction enzyme
* Buffer for restri… |
Restriction enzyme,
5'sticky |
| BamHI |
Recognition [GGATCC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X X X
* Restriction enzyme
* M.BamHI
* Buffer for restriction enz… |
Restriction enzyme,
5'sticky |
| BsmI |
Recognition [GAATGCN] Temp. 65 ºC BufferLowMidHighAcKCl ≥75% O O O X X
* Origin:Bacillus stearothermophilus NUB36
* Restriction enzy… |
Restriction enzyme,
3'sticky,
Not 37°C reaction |
| BstEII |
Recognition [GTNACC] Temp. 60 ºC BufferLowMidHighAcKCl ≥75% X X X O X
* Nucleotide N
* Origin:Bacillus stearothermophilus ET
* Rest… |
Restriction enzyme,
5'sticky,
Not 37°C reaction |
| DpnI |
DpnI cuts sites 'GATC' if the adenine is methylated.
Recognition [GATC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X X O X
Reaction
D… |
Restriction enzyme,
blunt |
| DraI |
Recognition [TTTAAA] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE… |
Restriction enzyme,
blunt |
| HaeII |
Recognition [RGCGCY] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O X O X
* GGCGCC/AGCGCT
* Nucleotide M,K
* Restriction enzyme
* Bu… |
Restriction enzyme,
3'sticky |
| HhaI |
Recognition [GCGC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X O O O X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE
… |
Restriction enzyme,
3'sticky |
| HincII |
Recognition [GTCGAC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% reactive X O O O X
* Restriction enzyme
* SalI
* AccI
* Buffer for re… |
Restriction enzyme,
blunt |
| HindIII |
Recognition [AAGCTT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X O X X X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE… |
Restriction enzyme,
5'sticky |
| KpnI |
Recognition [GGTACC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O X X O X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE… |
Restriction enzyme,
3'sticky |
| MboI |
Recognition [GATC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X O O O O
Isoscizomer
Sau3AI
Sau3AI is an isoscizomer of MboI. Sau3AI can … |
Restriction enzyme,
5'sticky |
| MspA1I |
Recognition [CMGCKG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X X X O X
* CAGCTG / CCGCGG
* Nucleotide M,K
* Restriction enzyme
* B… |
Restriction enzyme,
blunt |
| NarI |
Recognition [GGCGCC] Temp 37 °C BufferLowMidHighAcKCl ≥75% O X X X X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE
… |
Restriction enzyme,
5'sticky |
| NdeI |
Recognition [CATATG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X X O O X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE… |
Restriction enzyme,
5'sticky |
| NsiI |
Recognition [ATGCAT] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% X X O X X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE… |
Restriction enzyme,
3'sticky |
| PstI |
Recognition [CTGCAG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X X O X X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE… |
Restriction enzyme,
3'sticky |
| PvuI |
Recognition [CGATCG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X X O X O
with BSA
* Restriction enzyme
* Buffer for restriction enzymes… |
Restriction enzyme,
3'sticky |
| PvuII |
Recognition [CAGCTG] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O O O X O
* Restriction enzyme
* AluI
* MspA1I
* Buffer for restri… |
Restriction enzyme,
blunt |
| RsaI |
Recognition [GTAC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O O X O X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE
… |
Restriction enzyme,
blunt |
| SacI |
Recognition [GAGCTC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O X X O X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE… |
Restriction enzyme,
3'sticky |
| SalI |
Recognition [GTCGAC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% reactive X X O X X
* Restriction enzyme
* HincII
* AccI
* TaqI
* B… |
Restriction enzyme,
5'sticky |
| Sau3AI |
Recognition [GATC] Temp. 37 ºC BufferLowMidHighAcKCl ≥75% O O O O X
Isoscizomer
The recognition sites of isoscizomer, MboI will be blo… |
Restriction enzyme,
5'sticky |
| ScaI |
Recognition [AGTACT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% o o O X X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE… |
Restriction enzyme,
blunt |
| SmaI |
Recognition [CCCGGG] Temp. 25 ºC BufferLowMidHighAcKCl ≥75% X O O O X
* Restriction enzyme
* AvaI
* Buffer for restriction enzyme… |
Restriction enzyme,
blunt,
Not 37°C reaction |
| SphI |
Recognition [GCATGC] Temp. 37 °C BufferLowMidHighAcKCl ≥75% O O X O X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE… |
Restriction enzyme,
3'sticky |
| SspI |
Recognition [AATATT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X o X X X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE… |
Restriction enzyme,
blunt |
| Star activity |
It has been demonstrated that under extreme non-standard conditions, restriction endonucleases are capable of cleaving sequences which are similar but not ident… |
Restriction enzyme |
| StuI |
Recognition [AGGCCT] Temp. 37 °C BufferLowMidHighAcKCl ≥75% o O X o X
* Buffer for restriction enzymes
* REBASE
Restriction enzyme,
… |
Restriction enzyme,
blunt |
| TaqI |
Recognition [TCGA] Temperature 65 ºC BufferLowMidHighAcKCl ≥75% X X O O X
* Origin: Thermus aquaticus YTI
* Buffer for restriction en… |
Restriction enzyme,
5'sticky,
Not 37°C reaction |
| XbaI |
Recognition [TCTAGA] Temp. 37 °C BufferLowMidHighAcKCl ≥75% X O X X X
* Restriction enzyme
* Buffer for restriction enzymes
* REBASE… |
Restriction enzyme,
5'sticky |
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