| Page | Description | Tags |
| Ampicillin |
Ampicillin is an antibiotics that inhibits synthesis of cell-wall.
Resistance
* β-lactamase
* InterPro entry
Preparation
* Solve sodium ampicillin 5g i… |
antibiotics,
preparation |
| Buffer solutions |
Good's buffer
et al.
They have the following characters.
* They have physiological range of pKa(6.0 - 9.0).
* The pKa values of them are not influenced b… |
Buffer,
preparation |
| Chloramphenicol |
Chloramphenicol is an antibiotic that inhibits translation of protein from mRNA. It binds A site in a ribosome 50S subunit and inhibits peptidyltransferase acti… |
antibiotics,
preparation |
| Edetic acid |
Preparation
0.5M EDTA (pH 8.0)
EDTA-2Na-2 186.1 g700 mL (final 1L)
* Solve EDTA by adding NaOH pelette, and adjust pH to 8.0.
* Add to 1 L.
* Autoclav… |
preparation,
buffer |
| Ethidium bromide |
A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful … |
Preparation,
Toxin |
| G418 (Geneticin) |
G418 is an inhibitor of protein synthesis.
Molecular Weight 496.553 g/mol Molecular Formula
Resistance
* Neor/Kanr (Aminoglycoside phosphotransfera… |
antibiotics,
preparation |
| HEPES |
HEPES is one of the Goods' buffers.
Name HEPES Molecular Formula Molecular Weight 238.306CAS No. 7365-45-9 pKa(25ºC) 7.48
Preparation
1M HEPES-KOH
* Disol… |
buffer,
preparation |
| Kanamycin |
Kanamycin is an antibiotics that inhibits synthesis of protein by binding to 30S ribosome.
NameKanamycinMeSH mesh>68007612 Molecular Weight 484.499 g/mol Mol… |
antibiotics,
preparation |
| Lysozyme |
Preparation
5 mg/mL Lysozyme soln.
* Solve 50 mg of lysozyme in
* Add upto 10 mL.
* Dispense into 1 mL tubes.
* Store at -20 °C
:!:
* Lysozyme - … |
Enzyme,
Antibiotics,
Preparation |
| PCI extraction |
PCI (phenol-chloroform-isoamylalchol) extraction is applied to remove protein portion in the solution to extract nucleicacids.
Procedure
* Add PCI solution … |
preparation |
| Penicillin |
Penicillin G
A penicillin derivative commonly used in the form of its sodium or potassium salts in the treatment of a variety of infections. It is effective ag… |
antibiotics,
preparation |
| Phosphate buffer |
Phosphate buffer is often used for column chlomatography. But it is not an ideal buffer for biological assays because phosphate can act as either a substrate or… |
buffer,
preparation |
| Sodium acetate |
The trihydrate sodium salt of acetic acid, which is used as a source of sodium ions in solutions for dialysis and as a systemic and urinary alkalizer, diuretic,… |
salt,
preparation |
| Sodium acetate trihydrate |
3M NaOAc preparation
* Solve 204.1g (1.5 mol) of Sodium acetate trihydrate in approx. 400mL of
* Adjust pH by Acetic acid (pH 5.2)
* Add upto 500mL
* Ste… |
preparation |
| Streptomycin |
An antibiotic produced by the soil actinomycete Streptomyces griseus. It acts by inhibiting the initiation and elongation processes during protein synthesis. (M… |
Antibiotics,
Preparation |
| TAE buffer |
TAE buffer is Tris-acetate buffer containing EDTA. It is used for agarose gel electrophoreses.
Preparation
50x TAE
CompositionTris242 gAcetic acid57.1… |
Buffer,
Preparation |
| TBE buffer |
TBE buffer is Tris-borate buffer containing EDTA for gel electrophoreses.
Preparation
10x TBE
Mix the followings and adjust the volume to 1L.
Store at room … |
Buffer,
Preparation |
| TE buffer |
This solution is used for stock of DNA. EDTA is strong chelator of metal ions that are cofactors of endonucleases.
Preparation
* Mix two stock solution, 1M T… |
preparation,
buffer |
| Tween 20 |
Sorbitan mono-9-octadecanoate poly(oxy-1,2-ethanediyl) derivatives; complex mixtures of polyoxyethylene ethers used as emulsifiers or dispersing agents in pharm… |
Preparation,
Detergent |
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