| Page | Description | Tags |
| ADA (buffer) |
*
*
Name ADA Molecular Formula CAS26239-55-4pKa(25ºC)6.55
Name Adenosine deaminase ECbrendaec>3.5.4.4KEGG PathwayPurine metabolism
buffer |
buffer |
| Boric acid |
* TBE buffer
acid,
buffer |
acid,
buffer |
| Buffer solutions |
Good's buffer
et al.
They have the following characters.
* They have physiological range of pKa(6.0 - 9.0).
* The pKa values of them are not influenced b… |
Buffer,
preparation |
| CHES |
CHES is one of a Good's buffer.
Name CHES Molecular Formula CAS No.103-47-9pKa(20ºC)9.55
buffer |
buffer |
| Comparison between TAE and TBE |
There are some differences between TAE buffer and TBE buffer. Generally, 1xTAE or 0.5xTAE is used for agarose gel electrophoresis, whereas 1xTBE is used for PAG… |
Buffer |
| Edetic acid |
Preparation
0.5M EDTA (pH 8.0)
EDTA-2Na-2 186.1 g700 mL (final 1L)
* Solve EDTA by adding NaOH pelette, and adjust pH to 8.0.
* Add to 1 L.
* Autoclav… |
preparation,
buffer |
| HEPES |
HEPES is one of the Goods' buffers.
Name HEPES Molecular Formula Molecular Weight 238.306CAS No. 7365-45-9 pKa(25ºC) 7.48
Preparation
1M HEPES-KOH
* Disol… |
buffer,
preparation |
| MES |
buffer |
buffer |
| MOPS |
Name MOPS Molecular Weight 209.2633 g/mol Molecular Formula XLogP -1.2 pKa(25ºC) 7.17
buffer |
buffer |
| PIPES |
One of the Good's buffers with pKa of 7.48 (25ºC)。
buffer |
buffer |
| Phosphate buffer |
Phosphate buffer is often used for column chlomatography. But it is not an ideal buffer for biological assays because phosphate can act as either a substrate or… |
buffer,
preparation |
| Phosphate buffered saline |
* Disolve the following chemicals in approx. 800 mL of .
* NaCl 80 g
* KCl 2 g
*
*
Wikipedia
* Phosphate buffered saline
Phosphate buffered sali… |
buffer |
| TAE buffer |
TAE buffer is Tris-acetate buffer containing EDTA. It is used for agarose gel electrophoreses.
Preparation
50x TAE
CompositionTris242 gAcetic acid57.1… |
Buffer,
Preparation |
| TBE buffer |
TBE buffer is Tris-borate buffer containing EDTA for gel electrophoreses.
Preparation
10x TBE
Mix the followings and adjust the volume to 1L.
Store at room … |
Buffer,
Preparation |
| TE buffer |
This solution is used for stock of DNA. EDTA is strong chelator of metal ions that are cofactors of endonucleases.
Preparation
* Mix two stock solution, 1M T… |
preparation,
buffer |
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